Re: [Histonet] CD3 on BM
Are you using a mAb or pAb? We were using a mAb for awhile, but we
noticed that it did not do well on bone marrow specimens so we went back
to the pAb.
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
>>> eileen dusek 08/27/04 06:13AM >>>
Good Morning,
Is anyone having trouble with CD3 on BM? Our stain works great on
regular tissue but weak on BM. We pressure cook the slides and yesterday
I tried using an amp, still no staining. I was thinking of trying no
pretreatment or protease instead of PC. Are there any other
suggestions?
Sorry about the font change, I'm not sure how it happened.
I appreciate all your help
Eileen
Edward Hospital
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