This has been our experience as well.  Remember, you are working with
"malignant" cells so all bets are off in terms of protein expression. 
What type of pretreatment are you using for CD15?

Rich Cartun

>>> "Van Eyck, Deb"  08/15/02 04:04PM >>>


Hello everyone----Have a question for you in regards to CD15 - Leu MI 
- my
pathologist and I keep talking about this ----most all reed sternberg
cells
should stain with this antibody when they are present.  This doesn't
seem to
be the case in most preps even when my fixation and morphology is
great.
Does anyone have experience that produces this result?  I have great
staining in some cells--membrane and or great golgi apparatus----but
never
have all cells staining.   It would be nice to get them all to light
up!If
you do please share info, products, protocols, times etc.  your wisdom
would
be greatly appreciated.  How about incubation times  - I have found
extending this does help in some antibodies-not all!  Thanks f