The method is a modification of Seligman et al 1968, using DAB and 0.1M
phosphate buffer pH7.4.

-----Original Message-----
From: kwalters [mailto:kwalters@blue.weeg.uiowa.edu]
Sent: 27 August 2002 13:41
To: Andrew.Shand@north-bristol.swest.nhs.uk; histonet
Subject: RE: Cytochrome C


>===== Original Message From Andrew.Shand@north-bristol.swest.nhs.uk =====
>We have been using SIGMA Cytochrome C (product number C8266) from horse
>heart, on our frozen sections of muscle bxs for years and have been
notified
>it has been discontinued. Sigma suggest another product but it is a type 3
>Cytochrome C . The method specifies type 2. Does it make any difference?
Can
>you help and which product do you use?
>
>Bob Quilty
>Dept of Neuropathology
>Frenchay Hospital
>Bristol UK


Hi Bob,

What method are you using?

Kathy

Kathy Walters                                // 
Central Microscopy Research Facility        / /
85 EMRB                                    / /\
University of Iowa                        / /\ \
Iowa City, Iowa 52242                    / /  \ \
Phone #:  (319) 335-8142                / /    \ \
Fax #:  (319) 384-4469                ______   ((0))
email: Katherine-Walters@uiowa.edu     |__|    / /
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