Dear Histonetters,

We routinely process mouse embryos from day 7dpc and up. We have a request 
to process embryos that have been pre-stained with an alkaline phosphate 
substrate - INT BCIP. This substrate is alcohol soluble. Routine paraffin 
processing, no matter how short, reduces or eliminates the staining. Can 
anyone suggest alternative dehydrants that I can use? There are some 
methods that use highly toxic solvents, we would like to avoid these is 
possible.

Many thanks,

Keith Rogers
SAIC Frederick
(301-846-5190)