I often do all preparation the day before I do an IHC run and let the slides
sit in buffer overnight in the refridgerator. The only time I don't do this
is with tissue I feel may be prone to falling off and the long soak may
cause more tissue fall-off.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
Ms-G32
1600 Clifton Road
Atlanta, GA 30333
USA

PH: 404-639-3964
FAX: 404-639-3043

email: tim9@cdc.gov




-----Original Message-----
From: Louise Taylor [mailto:louiset@mail.saimr.wits.ac.za]
Sent: Tuesday, August 29, 2000 6:43 AM
To: histonet
Subject: Dewaxing slides


Hi histonetters

Has anyone had any experience with loss of antigenicity in slides that have
been dewaxed and either left soaking in water/buffer overnight or allowed to
airdry?

The reason I ask this is that we are moving to an automated IHC sytem in the
near future and will be dealing with a lot of slides and a short turn around
time.

Any input appreciated

Louise Taylor
Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa