You might try a water bath at 60-70 degrees overnight in whatever buffer you 
are using in the microwave.
Shirley Powell

>===== Original Message From Efrain Pacheco <epacheco2001@yahoo.com> =====
>Does anybody has any procedure to do HIER (heat
>induced epitope retrival)on bone tissue without
>loosing the cortical bone? I have two antibodies that
>appear to work well after microwave HIER except for
>the fact that the hard bone comes off the slide,
>apparently because of the heat. By the way, we are
>working with rat femurs. I will appreciate any
>suggestions.
>
>Efrain Pacheco, HT(ASCP)
>Histology Associate
>Amgen Inc.
>
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