Chris,
We also use the Benchmark XT and we routinely microwave process all of
our biopsy tissues. When we purchased our microwave processor, I ran a
large range of antibodies comparing routine processing vs microwave
processing (same tissues but processed differently) and found that the
all of the IHC tests performed on the microwave tissues were equal to or
better than the tissues routinely processed. 
Charlene


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Christine Jacobs
Sent: Wednesday, April 04, 2007 11:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave Processing and IHC's. .

We are currently using the Ventana Benchmark for IHC staining and are
thinking about bringing microwave processing into our lab. Does anyone
out there know of documentation concerning IHC staining on microwave
rpocessed tissue? Is anyone successfully staining IHC's on microwave
processed tissue? Did you have to re-validate your antibodies or simply
re-test your protocols on microwave processed tissue?
Any help with this issue would be greatly appreciated.
 
Chris Jacobs, HT(ASCP), QIHC
Histology Dept.
NorDx Labs
Scarborough, ME
jacobc@mmc.org
 
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