Dear Cathy,
You may want to use an anti-rat polymer for this double stain.
BIOCARE Medical makes a Rat 0n Mouse polymer in either HRP or ALP.
Please contact me for further information.
All the Best,
Linda Dean
Biocare Medical
Research Sales
(925) 603-8025
cell: (925)640-0074
Linda@biocare.net
BIOCARE MEDICAL LLC.
4040 Pike Lane
Concord, CA 94520
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Monday, April 02, 2007 10:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 41, Issue 2
Send Histonet mailing list submissions to
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Today's Topics:
1. Illinois Society for Histotechnologists Spring Meeting
(Rae Staskiewiez)
2. RE: Illinois Society for Histotechnologists Spring Meeting
(Thom Jensen)
3. Re: Re: Paper Bags (koellingr@comcast.net)
4. Double immunostaining with a rabbit and rat primary antibody
(Cathy Malcontenti-Wilson)
5. Prox1 / lymphatic endothelium (Mikael Niku)
6. Eosinophils (Marilyn Tyler)
7. RE: Peloris processor (Bartlett, Jeanine (CDC/CCID/NCZVED))
8. Glycoprotein 2B, 3A (Mildred Fail)
9. Safety survey (2) (Rene J Buesa)
10. Help in brain tisuue processing (Leila Ramos)
11. Peloris Processor (Judy Collins)
12. Re: Help in brain tisuue processing (Geoff McAuliffe)
13. Biopsy bags (Webb, Dorothy L)
14. FW: Job Opening (Walzer Susan)
15. Fw: [Histonet] Eosinophils (Ian Montgomery)
16. Re: Eosinophils (Geoff McAuliffe)
17. Please unsubscribe me (Adam Kirk)
18. unsubscrible (judi.ford@jax.org)
19. AW: [Histonet] Peloris Processor and isopropanol (Gudrun Lang)
20. Infectivity of FFPE tissue (Maray Weirauch)
----------------------------------------------------------------------
Message: 1
Date: Sun, 1 Apr 2007 12:36:42 -0500
From: "Rae Staskiewiez"
Subject: [Histonet] Illinois Society for Histotechnologists Spring
Meeting
To: "Histonet"
Cc: Renee Walker
Message-ID: <001401c77484$54c3c650$0302a8c0@rae1ktsbyhej72>
Content-Type: text/plain; charset="us-ascii"
If you are planning on attending the ISH 2007 Spring Meeting May
17-18,2007 in Bloomington, IL, the cutoff for room reservations is April 16,
2007. To make your reservation, call The Chateau at 309-662-2020. Room rates
are $89 + 12% tax per night. Mention that your are with the ISH when making
your reservations. To view the program go to www.ilhisto.org
and click on 2007 Spring Symposium. If you have
questions about the program, please contact Jane Chladny at
mchladny@uiuc.edu or call 217-333-8708. You may also register for the
meeting at www.ilhisto.org by clicking on event
registration. If you have questions about registration please contact
Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for
information at walker2@uiuc.edu or call 217-333-8708.
Rae Ann Staskiewicz
Site Chair
Illinois Society for Histotechnologists
------------------------------
Message: 2
Date: Sun, 01 Apr 2007 18:53:50 +0000
From: "Thom Jensen"
Subject: RE: [Histonet] Illinois Society for Histotechnologists Spring
Meeting
To: raestask@grics.net, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format=flowed
I don't know if you are having any workshops on Tissue Microarrays but I
would like you to know of instructional information at website:
arrayworkshop.com
It is free to all including instructional videos for constructing tissue
micro arrays.
Best wishes,
Thom Jensen
>From: "Rae Staskiewiez"
>To: "Histonet"
>CC: Renee Walker
>Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting
>Date: Sun, 1 Apr 2007 12:36:42 -0500
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>
> If you are planning on attending the ISH 2007 Spring Meeting
>May
>17-18,2007 in Bloomington, IL, the cutoff for room reservations is April
>16,
>2007. To make your reservation, call The Chateau at 309-662-2020. Room
>rates
>are $89 + 12% tax per night. Mention that your are with the ISH when making
>your reservations. To view the program go to www.ilhisto.org
> and click on 2007 Spring Symposium. If you have
>questions about the program, please contact Jane Chladny at
>mchladny@uiuc.edu or call 217-333-8708. You may also register for the
>meeting at www.ilhisto.org by clicking on event
>registration. If you have questions about registration please contact
>Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for
>information at walker2@uiuc.edu or call 217-333-8708.
>
>
>
>Rae Ann Staskiewicz
>
>Site Chair
>
>Illinois Society for Histotechnologists
>
>
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
The average US Credit Score is 675. The cost to see yours: $0 by Experian.
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------------------------------
Message: 3
Date: Mon, 02 Apr 2007 00:16:38 +0000
From: koellingr@comcast.net
Subject: Re: [Histonet] Re: Paper Bags
To: histonet@lists.utsouthwestern.edu
Message-ID:
<040220070016.21584.46104B66000A8E9D0000545022058844849D09020704040A0105@com
cast.net>
Content-Type: text/plain
To work with these kinds of specimens, for years and years in hospital used
Goody curler paper (sorry I don't know anything about hairdressers curling
hair but they can be had at drugstore/department store where they sell hair
products). Get 500 or so for a couple of dollars.
Wet them in the fixative. Mentally divide the paper into thirds each way so
you have a mental image of 9 square areas in a 3x3 array. Carefully put
your fragments or shavings or small biopsies in center area. Using forceps,
fold up the bottom third. Fold down top third. Fold in left hand and right
hand thirds to end up with square that fits in cassette and won't unfold.
When processed, while unfolding you know where those bits are. Either in
the center area or the single area folded over top of it.
It silly, its dirt cheap, its not high-tech, its not cool or sophisticated,
but for us in my lab, we never would loose any bits or pieces. Maybe we
were lucky with the set-up but if I put 8 minute fragments of a gastric
biopsy in, I felt confident and always could locate those 8 fragments easily
for embedding. Bags I'd have trouble in creases and corners and even too
with mesh cassettes. Processed well and any problem I thought might have
(like getting paper shreds into block for bad sectioning), just never
happened.
I still think those Goody curl papers are the best. I've never had them
fail to give me back every single bit of irreplaceable tissue I put in. And
pretty economically.
Raymond Koelling
PhenoPath Laboratories
Seattle, WA
-------------- Original message --------------
From: rsrichmond@aol.com
> Why would anyone want to use paper rather than the present-day nylon
> specimen bags?
>
> I recently looked at the Fisher Web site and was astonished to learn
> from the
>
> fisherhealthcare.com Web site, accessed a few days ago:
> biopsy bag 30 x 50 mm, Fisherbrand 15-182-116
> 500 bags for $228.17
> in contrast, Fisher offers biopsy foam pads, 22-038221
> 1000 rectangular pads for $73.91
>
> In other words, those nylon bags list at around 45 cents US each, while
> the little blue foam pads are around 7 cents each, or half that if you
> cut them in two as I usually do.
>
> Finding this out certainly made me change the way I use these two items
> - put small discrete biopsy specimens on blue pads (marked with a small
> drop of safranin solution), and reserve the bags for small curettage
> specimens, cell blocks, and things like that.
>
> I've used these two items in a good man pathology practices, but never
> knew the cost of them before, since catalogs are always locked up in
> the lab manager's office and not available to histotechnologists. Will
> some of you Good Managers enlighten me as to why it's Good Management
> Practice to have bench techs not know the cost of the items they work
> with?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> ________________________________________________________________________
> AOL now offers free email to everyone. Find out more about what's free
> from AOL at AOL.com.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 02 Apr 2007 14:40:59 +1000
From: Cathy Malcontenti-Wilson
Subject: [Histonet] Double immunostaining with a rabbit and rat
primary antibody
To: histonet@lists.utsouthwestern.edu
Message-ID:
<6.2.1.2.2.20070402142900.04427de8@mail.staff.unimelb.edu.au>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear All,
We wish to do a double immunostain for CD34 and active caspase 3 on
paraformaldehyde fixed mouse tissues. The cd34 primary is rat monoclonal
anti cd34 The caspase 3 primary is a rabbit polyclonal antibody.
We would like to use the Dako Envision G/2 kit for double staining, however
this kit is for mouse or rabbit primaries only. When we do a single stain
for cd34, we use a Rabbit anti Rat IgG as a linking antibody between the
primary and secondary antibody (Dako Envision plus kit for rabbit
primaries) and this works well on our tissues in our experience.
My question is: Can I add this linking antibody step to the method and
still use the Envision G/2 kit?
Does anyone have any experience with this sort of thing or any different
ideas on doing double staining using these particular antibodies?
All ideas and comment appreciated as always.
Cathy
------------------------------
Message: 5
Date: Mon, 02 Apr 2007 10:20:54 +0300
From: Mikael Niku
Subject: [Histonet] Prox1 / lymphatic endothelium
To: histonet@lists.utsouthwestern.edu
Message-ID: <4610AED6.1070208@helsinki.fi>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello!
I'm looking for a marker for lymphatic endothelium, which should have
wide species cross-reactivity, and works with paraffin-embedded,
formalin-fixed sections.
Chemicon/Upstate seems to have several promising products (two monos and
one polyclonal) but there's little information available on these.
Anyone have experience on these or other suitable antibodies?
With best regards,
Mikael Niku
--
////////////////////////////////////////////////////////////
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitdkv mieltd olen ldnsimaisesta sivistyksestd?
Minusta se olisi erinomainen ajatus!
- Gandhi
////////////////////////////////////////////////////////////
------------------------------
Message: 6
Date: Mon, 02 Apr 2007 12:09:43 +0200
From: "Marilyn Tyler"
Subject: [Histonet] Eosinophils
To: "histonet"
Message-ID: <4610F287.A78E.0090.0@uct.ac.za>
Content-Type: text/plain; charset=US-ASCII
Hi Again
Please could you tell me what tissue in mouse is a good positive
control for eosinophil staining. Thanks
Marilyn
------------------------------
Message: 7
Date: Mon, 2 Apr 2007 06:11:31 -0400
From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)"
Subject: RE: [Histonet] Peloris processor
To: "kristen arvidson" , "histonet"
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Please share with the whole group.
Thanks,
Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA 30333
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Saturday, March 31, 2007 6:21 PM
To: histonet
Subject: [Histonet] Peloris processor
Does anyone use the Peloris double chamber rapid tissue processor?
What's it like?
---------------------------------
Get your own web address.
Have a HUGE year through Yahoo! Small Business.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 02 Apr 2007 08:57:05 -0400
From: "Mildred Fail"
Subject: [Histonet] Glycoprotein 2B, 3A
To: , "Joyce Foster"
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Good morning,
We are looking for a lab to do this test. thank you in advance for
your help
Rena Fail
------------------------------
Message: 9
Date: Mon, 2 Apr 2007 06:07:27 -0700 (PDT)
From: Rene J Buesa
Subject: [Histonet] Safety survey (2)
To: histonet@lists.utsouthwestern.edu
Message-ID: <953403.9514.qm@web61224.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Colleagues:
Since 27 March when I posted about the "safety survey" 81 colleagues asked
and received the questionnaire. So far 49 have been returned which, by all
standards, is a very good response,but there are 32 still missing.
To those of you who received the questionnaire and have not answered it
yet, could you please fill it and send it back?
The more answers I get the more representative the results will be.
Thank you!
Reni J.
---------------------------------
Don't pick lemons.
See all the new 2007 cars at Yahoo! Autos.
------------------------------
Message: 10
Date: Mon, 2 Apr 2007 14:11:07 +0100
From: "Leila Ramos"
Subject: [Histonet] Help in brain tisuue processing
To: histonet@lists.utsouthwestern.edu
Message-ID:
<8978a9cf0704020611qd9dc075pf85ab472e090bfea@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear Mr. or Ms
We are working in a small lab from Canarias Island (Spain). We work with
goat brain with manual procedure, our problems are several in the
processing:
1. We obtain very dry blocks, when we try to cut with the microtome,
the tissues are fragile and it is impossible to obtain the correct
tissue.
2. When "we get proper block" (we can cut better the tissue), then we
try to put in the float, the tissue appears like a white colour in the
slide. After when we finish the stain process (hematoxyline-eosina and
kluver barrera both of them) the tissues are not attached on the glass
slides.
I explain you in fast way our protocol:
1. Fixation: the whole brain is embedded in Formol 10% (1 week
minimum)
2. Dehydration: 80: etanol 30 minutes; 96: 30 min x 2 times; 100 : 30
min x 5 times; xylene 1 hour x 2 times
3. Tissue embedding in paraffin. In cassetes with pure paraffin all
night- Only 1 step other times 2 changes in paraffin 1 hour
4. Cut with microtome at 8 um, slide with polylisine.
5. Stain process (Hematoxyline-Eosine and kluver barrera)
Thank a lot!!
Leila Ramos
Instituto de Investigacisn y Ciencias de Puerto del Rosario
------------------------------
Message: 11
Date: Mon, 2 Apr 2007 09:53:22 -0400
From: "Judy Collins"
Subject: [Histonet] Peloris Processor
To:
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
We have the Peloris rapid tissue processor. It is xylene free but does
not use microwaves. It uses isopropyl alcohol after the reagent alcohol
to remove all traces of water and then goes straight to the paraffin
from there. Amazingly, and contrary to everything we have been taught,
this works!
It processes small biopsies in approximately 1 hour. Shave biopsies
take approximately 2 hours and the larger specimens 4 to 6 hours. We
find the processing to be at least equal to routine processing and in
many cases, superior to it. Because it does not use xylene, the tissues
are not hard and brittle as they can sometimes be with routine
processing.
It has two separate retorts which can run two separate runs at the same
time. We are able to process our specimens throughout the day instead
of everything being processed at night as in the past. It has greatly
improved our TAT.
The Peloris tracks the number of blocks run and, based on this, tells
you when to change the various solutions. As a result, we have saved a
significant amount of money on reagents.
We are extremely happy with ours.
------------------------------
Message: 12
Date: Mon, 02 Apr 2007 10:36:07 -0400
From: Geoff McAuliffe
Subject: Re: [Histonet] Help in brain tisuue processing
To: Leila Ramos
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <461114D7.5060402@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Greetings Leila
If you are fixing the whole brain by immersion the interior morphology
won't be very good. I suggest cutting the brain into 1 cm slices and
fixing these for 1-2 weeks. You will be able to see enough of the
anatomy to know the order of the slices.
If you must process the brain whole you will need to fix by vascular
perfusion to see much (any) of the interior anatomy and your processing
will have to be much longer.
The rest of your schedule does not allow enough time for dehydration,
especially on a whole brain. The tissue does not stick to the slides
because it was not properly dehydrated so the paraffin does not infiltrate.
On the other hand, all night in hot paraffin is too long and produces
brittle tissue.
I suggest the following for 1 cm slices, not a whole goat brain.
After fixation, wash in water for several hours or overnight.
50% ethanol 1-2 hours. Agitation of the tissue duringhis and subsequent
steps is highly desirable.
70% ethanol 1-2 hours.
95% ethanol 1 hour.
100% ethanol 2 changes 1 hour each.
1:1 mix of 100% ethanol:xylene 1 hour.
Xylene, 2 changes 1 hour each.
3 changes of melted paraffin, under vacuum if possible, 45 min each.
Heat the wax only a few degrees above the melting point.
Embed
Leila Ramos wrote:
> Dear Mr. or Ms
> We are working in a small lab from Canarias Island (Spain). We work with
> goat brain with manual procedure, our problems are several in the
> processing:
>
> 1. We obtain very dry blocks, when we try to cut with the microtome,
> the tissues are fragile and it is impossible to obtain the correct
> tissue.
> 2. When "we get proper block" (we can cut better the tissue), then we
> try to put in the float, the tissue appears like a white colour in the
> slide. After when we finish the stain process (hematoxyline-eosina and
> kluver barrera both of them) the tissues are not attached on the glass
> slides.
>
> I explain you in fast way our protocol:
>
> 1. Fixation: the whole brain is embedded in Formol 10% (1 week
> minimum)
> 2. Dehydration: 80: etanol 30 minutes; 96: 30 min x 2 times; 100 : 30
> min x 5 times; xylene 1 hour x 2 times
> 3. Tissue embedding in paraffin. In cassetes with pure paraffin all
> night- Only 1 step other times 2 changes in paraffin 1 hour
> 4. Cut with microtome at 8 um, slide with polylisine.
> 5. Stain process (Hematoxyline-Eosine and kluver barrera)
>
> Thank a lot!!
>
> Leila Ramos
> Instituto de Investigacisn y Ciencias de Puerto del Rosario
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
------------------------------
Message: 13
Date: Mon, 02 Apr 2007 09:48:54 -0500
From: "Webb, Dorothy L"
Subject: [Histonet] Biopsy bags
To: Histonet@lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA2703640B56@hpes1.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
We use a fair amount of the biopsy bags and one can purchase them for
half the price that was quoted in the last histo-net. We purchase ours
from Thermo Fisher and still get them for $205.64 for 1000 bags. We do
not use the blue sponges due to the fact that we noticed reagent
carryover in our processor which compromised our quality of tissue
processing. Also, we have found that if one does not make certain the
mesh cassettes are "submerged" in formalin a piece of tissue may stick
to the top of the cassette or in a corner and not get proper processing.
That is why for now we have been utilizing the nylon bags and fine them
easy and efficient to use.
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
Regions Hospital is part of the HealthPartners family of care
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------------------------------
Message: 14
Date: Mon, 2 Apr 2007 10:56:45 -0400
From: "Walzer Susan"
Subject: [Histonet] FW: Job Opening
To: "Histonet \(E-mail\)"
Message-ID:
<471953BC63077941B82C26A4338272B42F050D@ORLEV03.hca.corpad.net>
Content-Type: text/plain; charset="iso-8859-1"
> -----Original Message-----
> From: Walzer Susan
> Sent: Wednesday, February 21, 2007 9:17 AM
> To: Histonet (E-mail)
> Subject: Job Opening
>
> We are looking for a histo-tech at St Pete. General Hospital. St Pete., FL
. Apply at:
>
> http://www.stpetegeneralhospital.com/
>
>
>
------------------------------
Message: 15
Date: Mon, 2 Apr 2007 16:17:06 +0100
From: "Ian Montgomery"
Subject: Fw: [Histonet] Eosinophils
To: "Histonet"
Message-ID: <004701c77539$fa0a1960$4724d182@ibls.gla.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
A mouse blood smear.
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.
----- Original Message -----
From: "Marilyn Tyler"
To: "histonet"
Sent: Monday, April 02, 2007 11:09 AM
Subject: [Histonet] Eosinophils
> Hi Again
> Please could you tell me what tissue in mouse is a good positive
> control for eosinophil staining. Thanks
> Marilyn
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 16
Date: Mon, 02 Apr 2007 11:27:47 -0400
From: Geoff McAuliffe
Subject: Re: [Histonet] Eosinophils
To: Marilyn Tyler
Cc: histonet
Message-ID: <461120F3.5030202@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Small intestine.
Geoff
Marilyn Tyler wrote:
>Hi Again
>Please could you tell me what tissue in mouse is a good positive
>control for eosinophil staining. Thanks
>Marilyn
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>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
------------------------------
Message: 17
Date: Mon, 2 Apr 2007 16:38:07 +0100
From: "Adam Kirk"
Subject: [Histonet] Please unsubscribe me
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
<40e76ce80704020838t79db3be3tb9510420fe659a08@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear Histonet, Please would you unsubscribe me from your mailing list?
Thanks.
Adam Kirk
--
Dr Adam Kirk MRCP
Renal/GIM Registrar
07966015047
------------------------------
Message: 18
Date: Mon, 2 Apr 2007 11:43:33 -0400 (EDT)
From: judi.ford@jax.org
Subject: [Histonet] unsubscrible
To: histonet@lists.utsouthwestern.edu
Message-ID:
<12180621.1175528613818.JavaMail.ocsadmin@jcs-mid-prod.jax.org>
Content-Type: text/plain; charset=ISO-8859-1
Please unsubscribe me from the histonet list.
Judi Ford
HIstotechnologist
------------------------------
Message: 19
Date: Mon, 2 Apr 2007 18:11:57 +0200
From: "Gudrun Lang"
Subject: AW: [Histonet] Peloris Processor and isopropanol
To:
Message-ID: <001801c77541$a382c990$6412a8c0@dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
I've seen a protocol for isopropanol-processing with an IPA-temperature
about 74 degrees. That is near the boilingpoint and means rather a boiling
out of the tissue than acting as intermedium. Similar protocols are found
with the microwave-techniques. I think there is no principal difference in
reaching the temperature.
What I'm a little confused about is the fact, that usually recommended
temperatures for tissueprocessing don't raise above 62 degrees. So how does
this influence staining, ihc-staining etc.?
What are the temperatures in your protocol?
What does the community think about this issue?
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Urspr|ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Judy
Collins
Gesendet: Montag, 02. April 2007 15:53
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Peloris Processor
We have the Peloris rapid tissue processor. It is xylene free but does
not use microwaves. It uses isopropyl alcohol after the reagent alcohol
to remove all traces of water and then goes straight to the paraffin
>from there. Amazingly, and contrary to everything we have been taught,
this works!
It processes small biopsies in approximately 1 hour. Shave biopsies
take approximately 2 hours and the larger specimens 4 to 6 hours. We
find the processing to be at least equal to routine processing and in
many cases, superior to it. Because it does not use xylene, the tissues
are not hard and brittle as they can sometimes be with routine
processing.
It has two separate retorts which can run two separate runs at the same
time. We are able to process our specimens throughout the day instead
of everything being processed at night as in the past. It has greatly
improved our TAT.
The Peloris tracks the number of blocks run and, based on this, tells
you when to change the various solutions. As a result, we have saved a
significant amount of money on reagents.
We are extremely happy with ours.
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------------------------------
Message: 20
Date: Mon, 02 Apr 2007 12:15:14 -0400
From: "Maray Weirauch"
Subject: [Histonet] Infectivity of FFPE tissue
To:
Message-ID:
Content-Type: text/plain; charset=US-ASCII
In the past, we have disposed of paraffin trimmings in regular trash and
old or broken, stained and unstained slides in non-biohazard glass
disposal unless they are from a suspected CJD case. We are reviewing
this procedure and cannot find documentation to support our policy, in
fact we have found some indication that TB may still be viable. What is
everyone else doing for disposal of their paraffin debris and slides for
disposal?
Thanks for your input!
------------------------------
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End of Histonet Digest, Vol 41, Issue 2
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