You might want to check for yourself.

Make up the amount you would like to use and run a control slide weekly
until signal fades. I would make sure all other lot numbers are the
same. I would think that time exposed to light may be a significant
factor. Just a thought.
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

Disclaimer: 
The information in this e-mail and any of its attachments is
confidential and may contain sensitive information. It should not be
used by anyone who is not the original intended recipient. If you have
received this e-mail in error please inform the sender and delete it
from your mailbox or any other storage devices. National Institute of
Allergy and Infectious Diseases shall not accept liability for any
statements made that are sender's own and not expressly made on behalf
of the NIAID by one of its representatives

-----Original Message-----
From: Gagnon, Eric [mailto:gagnone@KGH.KARI.NET] 
Sent: Tuesday, April 18, 2006 10:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Expiry Period - Immunofluorescence Dilutions

 
 
Hello Listers,
 
We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib.,
Kappa, Lambda, and C4d on renal biopsies, and the first four on skin
biopsies.  We are using Dako Antibody Diluting Buffer and mostly Dako
FITC antisera.
 
We currently make up about 1 ml of each, and the dilutions last us
between 1 and 3 weeks.  We would like to keep them longer if possible,
making them up in larger quantities, possibly with the goal of
transferring them to dispensers on our Ventana XT's.
]
Is there anyone doing the same immunofluorescence panel and dilutions,
and if so, how long are you able to use your dilutions once made?
 
Thanks for your assistance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet