Hello Listers,
 
We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib., Kappa, Lambda, and C4d on renal biopsies, and the first four on skin biopsies.  We are using Dako Antibody Diluting Buffer and mostly Dako FITC antisera.
 
We currently make up about 1 ml of each, and the dilutions last us between 1 and 3 weeks.  We would like to keep them longer if possible, making them up in larger quantities, possibly with the goal of transferring them to dispensers on our Ventana XT's.
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Is there anyone doing the same immunofluorescence panel and dilutions, and if so, how long are you able to use your dilutions once made?
 
Thanks for your assistance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada


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