Instead of post-mordanting in Zenker or saturated mercuric chloride, try
Bouin for 1 hour at 60 degree C., wash in running water 5-10 minutes to
remove the yellow, and then stain as usual with PTAH.

Usually works for us.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Cazares, Ruth" 
To: 
Sent: Friday, April 23, 2004 3:30 PM
Subject: [Histonet] PTAH Procedure


> Hello again Histonetters,
>
>
>
> I was wondering if anyone out there knows if there is a procedure for PTAH
> that does not require the tissue to be treated in Zenker's?  Any and all
> information will be greatly appreciated.  Thanks.
>
>
>
> Ruth Cazares
>
>
>
>
>
>
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