hello
i have prepared solochrome stain following this:
1 g of calcon (solochrome)
2 ml acetic acid glacial
98 ml distilled water
and let it for 3 months before use

i tried this stain on MMA sections of thin reconstructed and mineralized tissue, 
i put section of 50um in this stain for 1 hour, i didn't see anything stained on hot plate or not
could any one help me ? is the stain not good ? or something else ?
thank you very much for your help
Myriam 

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