As far as the relationship of mm Hg to ml/min with a perfusion pump -
aren't the innner diameter and stiffness of the tubing (and the i.d. of
the cannula) going to influence the final pressure, too? I don't think
adjusting final pump speed alone is going to accurately duplicate the
"hanging bag" pressure. Can you check the literature and see what other
groups have reported and work from there?

|--------------------------------------------------|
 Tamara Howard
 Department of Cell Biology and Physiology
 University of New Mexico - Health Sciences Center
 Albuquerque, NM 87131
 thoward@unm.edu
|--------------------------------------------------|


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