We have been successful with Dako's CD117 using it at 1:100 with out any treatment.
Our control tissue is a gastrointestinal stromal tumor.
I hope this helps.
Eileen C. Dusek
Central DuPage Hospital
>From: "Klassen, Shannon SDH"
>To: 'Histonet'
>Subject: c-kit (CD117)
>Date: Thu, 18 Apr 2002 09:55:52 -0600
>
>Can someone out there in histoland help me get this antibody working -
>Novocastra ckit clone T595 - I'm not getting membrane staining - some
>cytoplasmic and some almost appears nuclear. I've tried a variety of
>different tumors - GIST, melanoma, breast , normal skin and haven't had any
>success.
>Down to 1\10 with antigen retrieval in citrate buffer in microwave.
>HEELLLPPP!!
>
>Shannon Klassen
>IHC Tech
>Saskatoon Health District
>Saskatoon, Canada
>