Hi Jim
I have successfully stained mesothelioma's  on a Ventana Nexes using our
antibodies to Mesothelin and Calretinin.
Both require a high temp antigen retrieval in citrate buffer.
NCL-MESO 1/20
NCL-CALRETININ 1/40
16 Minute incubation
Basic DAB kit
If our antibody is one of those you have failed to achieve staining with
then  please contact me at  technical@novocastra .co.uk  and we will be
pleased to offer you assistance in your efforts to achieve satisfactory
staining
Paul Shore
Technical Services
Novocastra