Cryla,

Try turning the temperature down on your waterbath. It may be too hot.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> C H <cryla@hotmail.com> 21/April/2001 07:22am >>>
Hello, All

I wonder if any of you have a protocol that you wouldn't mind sharing for

auto-processing rat CNS tissue for paraffin embedding.  I am a novice at 
working with paraffin,using a Leica TP1050 and so far I have not found
the 
right combination of steps to prevent the tissue from overly expanding
and 
separating from the paraffin in the water bath.

Any assistance would be greatly appreciated.

Cyrla
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