-----Original Message-----
From: Shawn Griffin 
Sent: Friday, April 27, 2001 4:06 AM
To: 'Patti Loykasek'
Subject: RE: RCC


Patti,

We use RCC, clone 66.4c2 on paraffin tissue from NovoCastra.  We pretreat
the slides for 20 min using the steamer method.  We use everything from
Dako. The kit we are using is LSAB2HRP.  The dilution is 1:100 for 30
minutes.  I hope this will help you some.

Shawn Griffin
sgriffin@ameripath.com
Ameripath, Dallas

-----Original Message-----
From: Patti Loykasek [mailto:ploykasek@phenopath.com]
Sent: Thursday, April 26, 2001 5:11 PM
To: histonet
Subject: RCC


Can anyone recommend a RCC clone that works on FFPE tissue? Pretreatments?
We have tried several clones and pretreatments with inconsistent results.
Thanks for the help.

Patti Loykasek